Nowhere is the need for a truly ultra-inert base deactivated HPLC column more important than in high-throughput analysis or LC/MS. Even subtle differences in silanol activity between columns can markedly affect the chromatography in the very short, fast columns typically used. In addition, any peak tailing due to silanol activity can have a profound effect on detection limits in high sensitivity assays. ACE ultra-inert base deactivated HPLC columns virtually eliminate the negative effects of silanols in HPLC separations. This unequalled performance is now available in 20, 30, 35 and 50mm length columns, with diameters from 1.0 to 4.6mm i.d. The ACE columns are suitable for high throughput and LC/MS applications, and are the ideal choice for high volume screening assays used for drug analysis and combinatorial libraries where robust, reproducible columns are essential.
High performance – excellent peak shape for higher sensitivity
Choice of 13 low bleed phases for complete optimization
Ultra-inert silica enables MS compatible buffers to be used
20mm, 30mm, 35mm and 50mm column lengths
1.0, 2.1, 3.0, 4.0 and 4.6mm i.d.s
Reduce analysis time
ACE LC/MS and Rapid Analysis columns are available in a wide range of lengths and internal diameters, to enable complete method optimization. Reduced column lengths are used to increase productivity (see Figure 31c) whereas reducing column diameter improves sensitivity and reduces solvent waste (Figure 31d).